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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated MAGEB1 Polyclonal an (CL488-12400)

    IF Staining of HepG2 using CL488-12400

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 MAGEB1 antibody (CL488-12400) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated MAGEB1 Polyclonal an (CL488-12400)

    FC experiment of HepG2 using CL488-12400

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Mageb1 Polyclonal Antibody (CL488-12400)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite® Plus 488-conjugated MAGEB1 Polyclonal antibody

MAGEB1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-12400

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

MAGE Xp, MAGE B1 antigen, DSS-AHC critical interval MAGE superfamily 10, DAM10, CT3.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12400 targets MAGEB1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEB1 fusion protein Ag3094 相同性解析による交差性が予測される生物種
Full Name melanoma antigen family B, 1
Calculated molecular weight 347 aa, 39 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberBC013772
Gene Symbol MAGEB1
Gene ID (NCBI) 4112
RRIDAB_3672539
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43366
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAGEB1, also named as MAGEL1, MAGEXP, DAM10 and CT3.1, is a member of the MAGEB gene family. MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types and Testis/placenta, but not in other normal tissues. The MW of MAGEB1 is about 40-45kd.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MAGEB1 antibody CL488-12400Download protocol
FC protocol for CL Plus 488 MAGEB1 antibody CL488-12400Download protocol
Standard Protocols
Click here to view our Standard Protocols