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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAGI3 Recombinant antibody (84771-4-RR)

    WB analysis using 84771-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84771-4-RR (MAGI3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using MAGI3 Recombinant antibody (84771-4-RR)

    IF Staining of mouse brain using 84771-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using MAGI3 antibody (84771-4-RR, Clone: 242212C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84771-4-RR

    Affinity and Kinetic Characterization of 84771-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84771-4-RR against Human MAGI3 were performed. The affinity constant is 0.752 nM.

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MAGI3 Recombinant antibody

MAGI3 Uni-rAbTM Recombinant Antibody for WB, IF-P, ELISA
Cat No. 84771-4-RR
Clone No.242212C9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3, Membrane-associated guanylate kinase inverted 3, MAGI-3, KIAA1634, 242212C9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84771-4-RR targets MAGI3 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAGI3 fusion protein Ag18394 相同性解析による交差性が予測される生物種
Full Name membrane associated guanylate kinase, WW and PDZ domain containing 3
Calculated molecular weight 1481 aa, 163 kDa
Observed molecular weight140 kDa
GenBank accession numberBC130409
Gene Symbol MAGI3
Gene ID (NCBI) 260425
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ5TCQ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAGI proteins are scaffolding proteins, belonging to the Membrane-Associated Guanylate Kinase Inverted proteins of the MAGUK family. There are three members of the MAGI subfamily, MAGI-1, MAGI-2, and MAGI-3. They are comprised of 6 PDZ domains, 2 WW domains, and 1 GUK domain. They have been proven to mediate the transport and signal transduction of various G protein-coupled receptors (GPCRs) (PMID: 29625175). The antibody is specific to MAGI3.

Protocols

Product Specific Protocols
WB protocol for MAGI3 antibody 84771-4-RRDownload protocol
IF protocol for MAGI3 antibody 84771-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols