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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAN1B1 Monoclonal antibody, PBS Only

MAN1B1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C9F10

Cat no : 68121-1-PBS

Synonyms

ER alpha 1,2 mannosidase, ER mannosidase 1, ERMan1, MAN1B1, MANA ER

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MAN1B1 Monoclonal antibody (68121-1-Ig)

    WB analysis of HeLa using 68121-1-Ig (same clone as 68121-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68121-1-Ig (MAN1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAN1B1 Monoclonal antibody (68121-1-Ig)

    WB analysis using 68121-1-Ig (same clone as 68121-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68121-1-Ig (MAN1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAN1B1 Monoclonal antibody (68121-1-Ig)

    WB analysis using 68121-1-Ig (same clone as 68121-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68121-1-Ig (MAN1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68121-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MAN1B1 Monoclonal antibody (68121-1-Ig)

    IF Staining of U2OS using 68121-1-Ig (same clone as 68121-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using MAN1B1 antibody (68121-1-Ig, Clone: 3C9F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68121-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68121-1-PBS targets MAN1B1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MAN1B1 fusion protein Ag28302 相同性解析による交差性が予測される生物種
Full Name mannosidase, alpha, class 1B, member 1
Calculated molecular weight 80 kDa
Observed molecular weight80 kDa
GenBank accession numberBC002953
Gene symbol MAN1B1
Gene ID (NCBI) 11253
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MAN1B1, also known as ERMAN1, MANA-ER, belongs to the glycosyl hydrolase 47 family. MAN1B1 is involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation, specifically converting Man9GlcNAc to Man8GlcNAc isomer B. At high enzyme concentrations, it further trims the carbohydrates to Man5-6GlcNAc2 (PMID: 18003979). Mutations in MAN1B1 cause autosomal-recessive intellectual disability. This gene has been associated with Rafiq syndrome (PMID: 21763484).