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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAN1B1 Monoclonal antibody

MAN1B1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C9F10

Cat no : 68121-1-Ig

Synonyms

ER alpha 1,2 mannosidase, ER mannosidase 1, ERMan1, MAN1B1, MANA ER

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MAN1B1 Monoclonal antibody (68121-1-Ig)

    WB analysis of HeLa using 68121-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68121-1-Ig (MAN1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAN1B1 Monoclonal antibody (68121-1-Ig)

    WB analysis using 68121-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68121-1-Ig (MAN1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAN1B1 Monoclonal antibody (68121-1-Ig)

    WB analysis using 68121-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68121-1-Ig (MAN1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MAN1B1 Monoclonal antibody (68121-1-Ig)

    IF Staining of U2OS using 68121-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using MAN1B1 antibody (68121-1-Ig, Clone: 3C9F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, HEK-293 cells, LNCaP cells, HaCaT cells, U-87 MG cells, HepG2 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68121-1-Ig targets MAN1B1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MAN1B1 fusion protein Ag28302 相同性解析による交差性が予測される生物種
Full Name mannosidase, alpha, class 1B, member 1
Calculated molecular weight 80 kDa
Observed molecular weight80 kDa
GenBank accession numberBC002953
Gene symbol MAN1B1
Gene ID (NCBI) 11253
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAN1B1, also known as ERMAN1, MANA-ER, belongs to the glycosyl hydrolase 47 family. MAN1B1 is involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation, specifically converting Man9GlcNAc to Man8GlcNAc isomer B. At high enzyme concentrations, it further trims the carbohydrates to Man5-6GlcNAc2 (PMID: 18003979). Mutations in MAN1B1 cause autosomal-recessive intellectual disability. This gene has been associated with Rafiq syndrome (PMID: 21763484).

Protocols

Product Specific Protocols
WB protocol for MAN1B1 antibody 68121-1-IgDownload protocol
IF protocol for MAN1B1 antibody 68121-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols