CoraLite® Plus 488-conjugated MAN1B1 Monoclonal antibody
MAN1B1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3C9F10
Cat no : CL488-68121
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-68121 targets MAN1B1 in IF/ICC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MAN1B1 fusion protein Ag28302 相同性解析による交差性が予測される生物種 |
| Full Name | mannosidase, alpha, class 1B, member 1 |
| Calculated molecular weight | 80 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | BC002953 |
| Gene symbol | MAN1B1 |
| Gene ID (NCBI) | 11253 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAN1B1, also known as ERMAN1, MANA-ER, belongs to the glycosyl hydrolase 47 family. MAN1B1 is involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation, specifically converting Man9GlcNAc to Man8GlcNAc isomer B. At high enzyme concentrations, it further trims the carbohydrates to Man5-6GlcNAc2 (PMID: 18003979). Mutations in MAN1B1 cause autosomal-recessive intellectual disability. This gene has been associated with Rafiq syndrome (PMID: 21763484).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MAN1B1 antibody CL488-68121 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
