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Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using MAOB Monoclonal antibody (66107-1-Ig)

    WB analysis of fetal human brain using 66107-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MAOB Monoclonal antibody (66107-1-Ig)

    WB analysis of human brain using 66107-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MAOB Monoclonal antibody (66107-1-Ig)

    WB analysis of HeLa using 66107-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAOB Monoclonal antibody (66107-1-Ig)

    WB analysis using 66107-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using MAOB Monoclonal antibody (66107-1-Ig)

    WB analysis of human kidney using 66107-1-Ig

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using MAOB Monoclonal antibody (66107-1-Ig)

    IHC staining of human brain using 66107-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MAOB Monoclonal antibody (66107-1-Ig)

    IHC staining of human brain using 66107-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using MAOB Monoclonal antibody (66107-1-Ig)

    IF Staining of mouse kidney using 66107-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using MAOB antibody (66107-1-Ig, Clone: 2B12F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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MAOB Monoclonal antibody

MAOB Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66107-1-Ig
Clone No.2B12F3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

monoamine oxidase B, MAO-B, MAO B, EC:1.4.3.21, Amine oxidase [flavin-containing] B

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected infetal human brain tissue, human brain tissue, human kidney tissue, HeLa cells, HepG2 cells, HuH-7 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66107-1-Ig targets MAOB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAOB fusion protein Ag17918 相同性解析による交差性が予測される生物種
Full Name monoamine oxidase B
Calculated molecular weight 520 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC022494
Gene Symbol MAOB
Gene ID (NCBI) 4129
RRIDAB_2881506
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27338
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position(PMID:3387449).

Protocols

Product Specific Protocols
WB protocol for MAOB antibody 66107-1-IgDownload protocol
IHC protocol for MAOB antibody 66107-1-IgDownload protocol
IF protocol for MAOB antibody 66107-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols