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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAOB Monoclonal antibody, PBS Only

MAOB Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B12F3

Cat no : 66107-1-PBS

Synonyms

MAO B, MAOB, monoamine oxidase B, Monoamine oxidase type B

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using MAOB Monoclonal antibody (66107-1-Ig)

    WB analysis of fetal human brain using 66107-1-Ig (same clone as 66107-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MAOB Monoclonal antibody (66107-1-Ig)

    WB analysis of HeLa using 66107-1-Ig (same clone as 66107-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAOB Monoclonal antibody (66107-1-Ig)

    WB analysis using 66107-1-Ig (same clone as 66107-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using MAOB Monoclonal antibody (66107-1-Ig)

    WB analysis of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using MAOB Monoclonal antibody (66107-1-Ig)

    WB analysis of human kidney using 66107-1-Ig (same clone as 66107-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using MAOB Monoclonal antibody (66107-1-Ig)

    IHC staining of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using MAOB Monoclonal antibody (66107-1-Ig)

    IHC staining of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using MAOB Monoclonal antibody (66107-1-Ig)

    IF Staining of mouse brain using 66107-1-Ig (same clone as 66107-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAOB antibody (66107-1-Ig, Clone: 2B12F3 ) at dilution of 1:40 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using MAOB Monoclonal antibody (66107-1-Ig)

    IF Staining of mouse brain using 66107-1-Ig (same clone as 66107-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAOB antibody (66107-1-Ig, Clone: 2B12F3 ) at dilution of 1:40 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66107-1-PBS targets MAOB in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAOB fusion protein Ag17918 相同性解析による交差性が予測される生物種
Full Name monoamine oxidase B
Calculated molecular weight 520 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC022494
Gene symbol MAOB
Gene ID (NCBI) 4129
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position(PMID:3387449).