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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated MAP2 Monoclonal antibody

MAP2 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C3E6

Cat no : CL488-67015

Synonyms

MAP2C, MAP2B, MAP2A, MAP-2, MAP 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using CoraLite® Plus 488-conjugated MAP2 Monoclonal anti (CL488-67015)

    IF Staining of rat brain using CL488-67015

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 488 MAP2 antibody (CL488-67015, Clone: 1C3E6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Neuro-2a cells using CoraLite® Plus 488-conjugated MAP2 Monoclonal anti (CL488-67015)

    FC experiment of Neuro-2a using CL488-67015

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MAP2 (CL488-67015, Clone:1C3E6) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inrat brain tissue
Positive FC (Intra) detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67015 targets MAP2 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MAP2 fusion protein Ag11349 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein 2
Calculated molecular weight 200 kDa
GenBank accession numberBC038857
Gene symbol MAP2
Gene ID (NCBI) 4133
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in brain and has important role in neuronal morphogenesis. Multiple high molecular weight (MW) and low molecular weight (MW) MAP2 isoforms are expressed within axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally specific manner. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MAP2 antibody CL488-67015Download protocol
FC protocol for CL Plus 488 MAP2 antibody CL488-67015Download protocol
Standard Protocols
Click here to view our Standard Protocols