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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated MEK2 Monoclonal antibody

MEK2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H8C2

Cat no : CL488-67410

Synonyms

ERK activator kinase 2, FLJ26075, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2, MAPKK2, MEK 2, MEK2, MKK2, PRKMK2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated MEK2 Monoclonal anti (CL488-67410)

    IF Staining of HeLa using CL488-67410

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 MAP2K2 antibody (CL488-67410, Clone: 1H8C2 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67410 targets MEK2 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MEK2 fusion protein Ag29871 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 2
Calculated molecular weight 44 kDa
Observed molecular weight 44-47 kDa
GenBank accession numberBC018645
Gene symbol MEK2
Gene ID (NCBI) 5605
RRIDAB_2919466
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MEK2 antibody CL488-67410Download protocol
Standard Protocols
Click here to view our Standard Protocols