• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MAP2K3 Recombinant antibody (80137-1-RR)

    WB analysis using 80137-1-RR (same clone as 80137-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using MAP2K3 Recombinant antibody (80137-1-RR)

    WB analysis of HepG2 using 80137-1-RR (same clone as 80137-1-PBS)

    WB result of MAP2K3 antibody (80137-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP2K3 transfected HepG2 cells. This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAP2K3 Recombinant antibody (80137-1-RR)

    WB analysis using 80137-1-RR (same clone as 80137-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using MAP2K3 Recombinant antibody (80137-1-RR)

    WB analysis of HT-1080 using 80137-1-RR (same clone as 80137-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of zebrafish tissue using MAP2K3 Recombinant antibody (80137-1-RR)

    WB analysis of zebrafish using 80137-1-RR (same clone as 80137-1-PBS)

    zebrafish tissue were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using MAP2K3 Recombinant antibody (80137-1-RR)

    WB analysis of HepG2 using 80137-1-RR (same clone as 80137-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MAP2K3 Recombinant antibody (80137-1-RR)

    WB analysis using 80137-1-RR (same clone as 80137-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAP2K3 Recombinant antibody (80137-1-RR)

    IF Staining of HeLa using 80137-1-RR (same clone as 80137-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAP2K3 antibody (80137-1-RR, Clone: 3O10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue) This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

MAP2K3 Recombinant antibody, PBS Only

MAP2K3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 80137-1-PBS
Clone No.3O10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

MEK3, MAPKK 3, MAPK/ERK kinase 3, MAP kinase kinase 3, EC:2.7.12.2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80137-1-PBS targets MAP2K3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 3
Calculated molecular weight 347 aa, 39 kDa
Observed molecular weight39 kDa
GenBank accession numberBC032478
Gene Symbol MAP2K3
Gene ID (NCBI) 5606
RRIDAB_2918868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP46734
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MAP2K3, also named MKK3, is a member of dual specificity kinase group and serves as a specific activator of p38 MAPK with MKK6. MKK3 can be phosphorylated by cytokines and environmental stress at sites Ser189 and Thr193 by MKKK proteins (MEKK 1-4). MKK3 plays a key role in cell differentiation, motility, division and death. It has been reported that MKK3 targeting may represent an effective and potentially safe therapeutic strategy to selectively kill cancer cells in colorectal cancer patients. (PMID: 31695024, 30770795, 26805700, 8622669)