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  • Featured Product
  • KD/KO Validated

MAP2K3 Recombinant antibody

MAP2K3 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat, Zebrafish

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3O10

Cat no : 80137-1-RR

Synonyms

MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MAPKK3, MEK 3, MEK3, MKK3, PRKMK3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAP2K3 Recombinant antibody (80137-1-RR)

    WB analysis using 80137-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MAP2K3 Recombinant antibody (80137-1-RR)

    WB analysis of HepG2 using 80137-1-RR

    WB result of MAP2K3 antibody (80137-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP2K3 transfected HepG2 cells.

  • Western Blot (WB) analysis of zebrafish tissue using MAP2K3 Recombinant antibody (80137-1-RR)

    WB analysis of zebrafish using 80137-1-RR

    zebrafish tissue were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MAP2K3 Recombinant antibody (80137-1-RR)

    WB analysis of HepG2 using 80137-1-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAP2K3 Recombinant antibody (80137-1-RR)

    WB analysis using 80137-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAP2K3 Recombinant antibody (80137-1-RR)

    WB analysis using 80137-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using MAP2K3 Recombinant antibody (80137-1-RR)

    WB analysis of HT-1080 using 80137-1-RR

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAP2K3 Recombinant antibody (80137-1-RR)

    IF Staining of HeLa using 80137-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAP2K3 antibody (80137-1-RR, Clone: 3O10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue)

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Tested Applications

Positive WB detected inC6 cells, HepG2 cells, Jurkat cells, A431 cells, HT-1080 cells, zebrafish tissue, C2C12 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80137-1-RR targets MAP2K3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Zebrafish samples.

Tested Reactivity Human, Mouse, Rat, Zebrafish
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 3
Calculated molecular weight 347 aa, 39 kDa
Observed molecular weight39 kDa
GenBank accession numberBC032478
Gene symbol MAP2K3
Gene ID (NCBI) 5606
RRIDAB_2918868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAP2K3, also named as MKK3, is a member of dual specificity kinase group and serves as a specific activator of p38 MAPK with MKK6. MKK3 can be phosphorylated by cytokines and environmental stress at sites Ser189 and Thr193 by MKKK proteins (MEKK 1-4). MKK3 plays a key role in cell differentiation, motility, division and death. It has been reported that MKK3 targeting may represent an effective and potentially safe therapeutic strategy to selectively kill cancer cells in colorectal cancer patients. (PMID: 31695024, 30770795, 26805700, 8622669)

Protocols

Product Specific Protocols
WB protocol for MAP2K3 antibody 80137-1-RRDownload protocol
IF protocol for MAP2K3 antibody 80137-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols