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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated MAP2K4 Monoclonal antibody

MAP2K4 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2E4C1

Cat no : CL594-67333

Synonyms

MEK4, JNK-activating kinase 1, JNK activating kinase 1, EC:2.7.12.2, Dual specificity mitogen-activated protein kinase kinase 4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated MAP2K4 Monoclonal antibody (CL594-67333)

    IF Staining of HepG2 using CL594-67333

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 MAP2K4 antibody (CL594-67333, Clone: 2E4C1 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated MAP2K4 Monoclonal antibody (CL594-67333)

    FC experiment of HepG2 using CL594-67333

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MAP2K4 (CL594-67333, Clone:2E4C1) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67333 targets MAP2K4 in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MAP2K4 fusion protein Ag11348 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 4
Calculated molecular weight 399 aa, 44 kDa
Observed molecular weight44-50 kDa
GenBank accession numberBC036032
Gene symbol MAP2K4
Gene ID (NCBI) 6416
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEK4 is also named as JNKK1, MAP2K4 , MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MEK4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL594 MAP2K4 antibody CL594-67333Download protocol
FC protocol for CL594 MAP2K4 antibody CL594-67333Download protocol
Standard Protocols
Click here to view our Standard Protocols