Featured Product
KD/KO Validated

MEK6 Polyclonal antibody

Name: MEK6 Polyclonal antibody
Brand: Proteintech
SKU: 12745-1-AP

MEK6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12745-1-AP

Synonyms

MAP kinase kinase 6, MAP2K6, MAPK/ERK kinase 6, MAPKK 6, MAPKK6, MEK 6, MEK6, MKK6, PRKMK6, SAPKK3

Validation Data Gallery

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AllWBIHC

WB analysis of HeLa using 12745-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12745-1-AP (MEK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12745-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12745-1-AP (MEK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 12745-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12745-1-AP (MEK6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12745-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12745-1-AP (MEK6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 12745-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, human kidney tissue
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below

Product Information

12745-1-AP targets MEK6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MEK6 fusion protein Ag3706 相同性解析による交差性が予測される生物種
Full Name	mitogen-activated protein kinase kinase 6
Calculated molecular weight	334 aa, 37 kDa
Observed molecular weight	37 kDa
GenBank accession number	BC012009
Gene symbol	MEK6
Gene ID (NCBI)	5608
RRID	AB_2141563
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for MEK6 antibody 12745-1-AP	Download protocol
IHC protocol for MEK6 antibody 12745-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Food Funct L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice. Authors - Kehong Liu View Article
human	WB	Sci Rep The ubiquitin ligase HERC1 regulates cell migration via RAF-dependent regulation of MKK3/p38 signaling. Authors - Leonardo Pedrazza View Article
	WB	Nat Aging Skin chronological aging drives age-related bone loss via secretion of cystatin-A Authors - Wenquan Liang View Article
human	WB	Acta Pharmacol Sin Targeting PRSS23 with tipranavir induces gastric cancer stem cell apoptosis and inhibits growth of gastric cancer via the MKK3/p38 MAPK-IL24 pathway Authors - Ji-Xian Xiong KD Validated View Article
human	WB	Cell Cycle SAMHD1, positively regulated by KLF4, suppresses the proliferation of gastric cancer cells through MAPK p38 signaling pathway Authors - Zhangming Chen View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MEK6 Polyclonal antibody

MEK6 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of HeLa using 12745-1-AP

WB analysis of human kidney using 12745-1-AP

IHC staining of human breast cancer using 12745-1-AP

IHC staining of human breast cancer using 12745-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice.

The ubiquitin ligase HERC1 regulates cell migration via RAF-dependent regulation of MKK3/p38 signaling.

Skin chronological aging drives age-related bone loss via secretion of cystatin-A

Targeting PRSS23 with tipranavir induces gastric cancer stem cell apoptosis and inhibits growth of gastric cancer via the MKK3/p38 MAPK-IL24 pathway

SAMHD1, positively regulated by KLF4, suppresses the proliferation of gastric cancer cells through MAPK p38 signaling pathway