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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated MEK6 Polyclonal antibody

MEK6 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-12745

Synonyms

MAP2K6, MKK6, MAPKK 6, MAPK/ERK kinase 6, MAP kinase kinase 6

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated MEK6 Polyclonal anti (CL647-12745)

    FC experiment of HeLa using CL647-12745

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human MEK6 (CL647-12745) (red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-12745 targets MEK6 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEK6 fusion protein Ag3706 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 6
Calculated molecular weight 334 aa, 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC012009
Gene symbol MEK6
Gene ID (NCBI) 5608
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 MEK6 antibody CL647-12745Download protocol
Standard Protocols
Click here to view our Standard Protocols