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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAP4 Monoclonal antibody, PBS Only

MAP4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C1A6

Cat no : 68271-1-PBS

Synonyms

Microtubule-associated protein 4, MAP-4, MAP 4, 1C1A6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAP4 Monoclonal antibody (68271-1-Ig)

    WB analysis using 68271-1-Ig (same clone as 68271-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68271-1-Ig (MAP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68271-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAP4 Monoclonal antibody (68271-1-Ig)

    IHC staining of human lung cancer using 68271-1-Ig (same clone as 68271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68271-1-Ig (MAP4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68271-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAP4 Monoclonal antibody (68271-1-Ig)

    IHC staining of human lung cancer using 68271-1-Ig (same clone as 68271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68271-1-Ig (MAP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68271-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MAP4 Monoclonal antibody (68271-1-Ig)

    IF Staining of A431 using 68271-1-Ig (same clone as 68271-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using MAP4 antibody (68271-1-Ig, Clone: 1C1A6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68271-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68271-1-PBS targets MAP4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAP4 fusion protein Ag1741 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein 4
Calculated molecular weight 121 kDa
Observed molecular weight210-240 kDa
GenBank accession numberBC012794
Gene symbol MAP4
Gene ID (NCBI) 4134
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)