MAP4 Monoclonal antibody
MAP4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1C1A6
Cat no : 68271-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | NCCIT cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68271-1-Ig targets MAP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | MAP4 fusion protein Ag1741 相同性解析による交差性が予測される生物種 |
Full Name | microtubule-associated protein 4 |
Calculated molecular weight | 121 kDa |
Observed molecular weight | 210-240 kDa |
GenBank accession number | BC012794 |
Gene symbol | MAP4 |
Gene ID (NCBI) | 4134 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)
Protocols
Product Specific Protocols | |
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WB protocol for MAP4 antibody 68271-1-Ig | Download protocol |
IHC protocol for MAP4 antibody 68271-1-Ig | Download protocol |
IF protocol for MAP4 antibody 68271-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |