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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated MAP4 Monoclonal anti (CL488-68271)

    IF Staining of A431 using CL488-68271

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite® Plus 488 MAP4 antibody (CL488-68271, Clone: 1C1A6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated MAP4 Monoclonal anti (CL488-68271)

    IF Staining of A431 using CL488-68271

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite® Plus 488 MAP4 antibody (CL488-68271, Clone: 1C1A6 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated MAP4 Monoclonal antibody

MAP4 Monoclonal Antibody for IF/ICC
Cat No. CL488-68271
Clone No.1C1A6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68271 targets MAP4 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAP4 fusion protein Ag1741 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein 4
Calculated molecular weight 121 kDa
Observed molecular weight210-240 kDa
GenBank accession numberBC012794
Gene Symbol MAP4
Gene ID (NCBI) 4134
RRIDAB_3672985
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP27816
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MAP4 antibody CL488-68271Download protocol
Standard Protocols
Click here to view our Standard Protocols