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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using MAP7 Polyclonal antibody (13446-1-AP)

    WB analysis of A549 using 13446-1-AP (same clone as 13446-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using MAP7 Polyclonal antibody (13446-1-AP)

    WB analysis of A431 using 13446-1-AP (same clone as 13446-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MAP7 Polyclonal antibody (13446-1-AP)

    WB analysis of HeLa using 13446-1-AP (same clone as 13446-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using MAP7 Polyclonal antibody (13446-1-AP)

    IP experiment of HeLa using 13446-1-AP (same clone as 13446-1-PBS)

    IP result of anti-MAP7 (IP:13446-1-AP, 4ug; Detection:13446-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using MAP7 Polyclonal antibody (13446-1-AP)

    IHC staining of human colon using 13446-1-AP (same clone as 13446-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13446-1-AP (MAP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAP7 Polyclonal antibody (13446-1-AP)

    IF Staining of HeLa using 13446-1-AP (same clone as 13446-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MAP7 antibody (13446-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MAP7 Polyclonal antibody (13446-1-AP)

    IF Staining of HepG2 using 13446-1-AP (same clone as 13446-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using MAP7 antibody 13446-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAP7 Polyclonal antibody (13446-1-AP)

    IF Staining of HeLa using 13446-1-AP (same clone as 13446-1-PBS)

    Immunofluorescent analysis of Hela cells, using MAP7 antibody 13446-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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MAP7 Polyclonal antibody, PBS Only

MAP7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13446-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Microtubule-associated protein 7, MAP-7, Epithelial microtubule-associated protein of 115 kDa, Ensconsin, E-MAP-115

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13446-1-PBS targets MAP7 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP7 fusion protein Ag4276 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein 7
Calculated molecular weight 749 aa, 84 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC025777
Gene Symbol MAP7
Gene ID (NCBI) 9053
RRIDAB_10644485
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14244
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.