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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®555-conjugated MARCKS Polyclonal antibody

MARCKS Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-20661

Synonyms

MARCS, MACS, 80K-L protein, 80KL, 80K L protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®555-conjugated MARCKS Polyclonal antibody (CL555-20661)

    IF Staining of mouse brain using CL555-20661

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®555 MARCKS antibody (CL555-20661) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated MARCKS Polyclonal antibody (CL555-20661)

    FC experiment of HeLa using CL555-20661

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human MARCKS (CL555-20661) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-20661 targets MARCKS in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myristoylated alanine-rich protein kinase C substrate
Calculated molecular weight 32 kDa
Observed molecular weight 80 kDa
GenBank accession numberNM_002356
Gene symbol MARCKS
Gene ID (NCBI) 4082
RRIDAB_2919638
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MARCKS, also named as MACS, PRKCSL and 80K-L protein, belongs to the MARCKS family. MARCKS is the most prominent cellular substrate for protein kinase C. It binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Protocols

Product Specific Protocols
IF protocol for CL555 MARCKS antibody CL555-20661Download protocol
FC protocol for CL555 MARCKS antibody CL555-20661Download protocol
Standard Protocols
Click here to view our Standard Protocols