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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated MAT1A Monoclonal ant (CL488-67408)

    IF Staining of HeLa using CL488-67408

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 MAT1A antibody (CL488-67408, Clone: 2F1C1 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated MAT1A Monoclonal ant (CL488-67408)

    FC experiment of HeLa using CL488-67408

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated MAT1A Monoclonal antibody (CL488-67408, Clone:2F1C1)(red), or 0.4 ug Isotype Control (CL488-66360-2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated MAT1A Monoclonal antibody

MAT1A Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67408
Clone No.2F1C1

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

MAT I/III, MAT 1, MAT, EC:2.5.1.6, AMS1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67408 targets MAT1A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MAT1A fusion protein Ag3076 相同性解析による交差性が予測される生物種
Full Name methionine adenosyltransferase I, alpha
Calculated molecular weight 395 aa, 44 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC018359
Gene Symbol MAT1A
Gene ID (NCBI) 4143
RRIDAB_3672946
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00266
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Methionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MAT1A antibody CL488-67408Download protocol
FC protocol for CL Plus 488 MAT1A antibody CL488-67408Download protocol
Standard Protocols
Click here to view our Standard Protocols