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- KD/KO Validated
CoraLite® Plus 488-conjugated MAVS; VISA Polyclonal antibody
MAVS; VISA Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-14341
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-14341 targets MAVS; VISA in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MAVS; VISA fusion protein Ag5655 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial antiviral signaling protein |
| Calculated molecular weight | 57 kDa |
| Observed molecular weight | 50-55 kDa, 70-75 kDa |
| GenBank accession number | BC044952 |
| Gene symbol | MAVS |
| Gene ID (NCBI) | 57506 |
| RRID | AB_2883082 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MAVS; VISA antibody CL488-14341 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility |
