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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated MBNL1 Monoclonal antibody

MBNL1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H2C12

Cat no : CL594-66837

Synonyms

DKFZp686P06174, EXP, EXP35, EXP40, EXP42, KIAA0428, MBNL, MBNL1, muscleblind like (Drosophila), Muscleblind like protein 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite®594-conjugated MBNL1 Monoclonal antibody (CL594-66837)

    IF Staining of A431 using CL594-66837

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®594 MBNL1 antibody (CL594-66837, Clone: 1H2C12 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66837 targets MBNL1 in IF/ICC applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MBNL1 fusion protein Ag7752 相同性解析による交差性が予測される生物種
Full Name muscleblind-like (Drosophila)
Calculated molecular weight 42 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC050535
Gene symbol MBNL1
Gene ID (NCBI) 4154
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MBNL1 (muscleblind-like 1) is one of the MBNL proteins containing 3 members (MBNL1-3) in vertebrates. And MBNL1 is widely expressed in adult tissues including brain, heart and skeletal muscle (PMID:25274774). As a highly conserved RNA-binding protein, MBNL1 regulates alternative splicing, alternative polyadenylation, mRNA stability and mRNA localization in the nucleus. The neurological diseases DM1 and DM2 (myotonic dystrophy type 1 and type2) are caused by expansion of repetitive sequences in non-coding regions in the DMPK gene because of the inappropriate patterns of alternative splicing owing to MBNL1(PMID:24354850). The antibody could recognize the common full-length protein in applications.

Protocols

Product Specific Protocols
IF protocol for CL594 MBNL1 antibody CL594-66837Download protocol
Standard Protocols
Click here to view our Standard Protocols