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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated MCL1 Monoclonal antibody

MCL1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E3C2

Cat no : CL594-66026

Synonyms

EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated MCL1 Monoclonal antibody (CL594-66026)

    IF Staining of HeLa using CL594-66026

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 MCL1 antibody (CL594-66026, Clone: 1E3C2 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-66026 is MCL1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MCL1 fusion protein Ag10609 相同性解析による交差性が予測される生物種
Full Name myeloid cell leukemia sequence 1 (BCL2-related)
Calculated molecular weight 350 aa, 37 kDa
GenBank accession numberBC107735
Gene symbol MCL1
Gene ID (NCBI) 4170
RRIDAB_2919938
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target.

Protocols

Product Specific Protocols
IF protocol for CL594 MCL1 antibody CL594-66026Download protocol
Standard Protocols
Click here to view our Standard Protocols