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Validation Data Gallery

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  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite®594-conjugated MCM5 Monoclonal antibody (CL594-67049)

    FC experiment of MCF-7 using CL594-67049

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MCM5 (CL594-67049, Clone:3B7F8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CoraLite®594-conjugated MCM5 Monoclonal antibody

MCM5 Monoclonal Antibody for FC (Intra)
Cat No. CL594-67049
Clone No.3B7F8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Synonyms

CDC46, CDC46 homolog, MCM5, P1 CDC46

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67049 targets MCM5 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MCM5 fusion protein Ag28704 相同性解析による交差性が予測される生物種
Full Name minichromosome maintenance complex component 5
Calculated molecular weight 82 kDa
Observed molecular weight 82-88 kDa
GenBank accession numberBC000142
Gene Symbol MCM5
Gene ID (NCBI) 4174
RRIDAB_3084743
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP33992
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.

Protocols

Product Specific Protocols
FC protocol for CL594 MCM5 antibody CL594-67049Download protocol
Standard Protocols
Click here to view our Standard Protocols