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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated MCM7 Monoclonal antibody

MCM7 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G1F6

Cat no : CL488-67446

Synonyms

CDABP0042, CDC47, CDC47 homolog, MCM2, MCM7, P1.1 MCM3, P1CDC47, P85MCM, PNAS 146

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated MCM7 Monoclonal anti (CL488-67446)

    IF Staining of HepG2 using CL488-67446

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 MCM7 antibody (CL488-67446, Clone: 1G1F6 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67446 targets MCM7 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MCM7 fusion protein Ag28558 相同性解析による交差性が予測される生物種
Full Name minichromosome maintenance complex component 7
Calculated molecular weight 81 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC013375
Gene symbol MCM7
Gene ID (NCBI) 4176
RRIDAB_2919474
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MCM7, also named as CDC47 homolog or P1.1-MCM3, is a 719 amino acid protein, which belongs to the MCM family. MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. the MCM2-7 complex participates in the pre-replication complex formation and exhibits helicase activity which makes DNA unwind, and resulting in recruitment of DNA polymerases and initiation of DNA replication and elongation [PMID: 29463213 ]. An immunohistochemical study has shown that MCM7 is increased in HCC. Silencing of MCM7 with shRNA inhibits the malignant behavior of HCC cells via cell cycle arrest and apoptosis [[PMID: 29463213].

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MCM7 antibody CL488-67446Download protocol
Standard Protocols
Click here to view our Standard Protocols