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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Mouse MCP-1/CCL2 Rabbit Recombinant Antibody

MCP-1/CCL2 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

230483A9

Cat no : CL647-98028

Synonyms

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of RAW 264.7 cells using CoraLite® Plus 647 Anti-Mouse MCP-1 Rabbit Recombi (CL647-98028)

    FC experiment of RAW 264.7 using CL647-98028

    1x10^6 untreated or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse MCP-1 Rabbit Recombinant Antibody (CL647-98028, Clone:230483A9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of RAW 264.7 cells using CoraLite® Plus 647 Anti-Mouse MCP-1 Rabbit Recombi (CL647-98028)

    FC experiment of RAW 264.7 using CL647-98028

    1x10^6 LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse MCP-1 Rabbit Recombinant Antibody (CL647-98028, Clone:230483A9)(red) or Isotype Control (blue). 1x10^6 untreated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse MCP-1 Rabbit Recombinant Antibody (CL647-98028, Clone:230483A9) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inLPS and Brefeldin A treated RAW 264.7 cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL647-98028 is MCP-1/CCL2 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 2
GenBank accession numberNM-011333
Gene symbol MCP-1/CCL2
Gene ID (NCBI) 20296
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Monocyte chemotactic protein 1 (MCP-1; also known as CCL2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 MCP-1/CCL2 antibody CL647-98028Download protocol
Standard Protocols
Click here to view our Standard Protocols