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  • Featured Product
  • KD/KO Validated

MCPH/BRIT1 Polyclonal antibody, PBS Only

MCPH/BRIT1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11962-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MCPH/BRIT1 Polyclonal antibody (11962-1-AP)

    WB analysis of HeLa using 11962-1-AP (same clone as 11962-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11962-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDA-MB-453s cells using MCPH/BRIT1 Polyclonal antibody (11962-1-AP)

    WB analysis of MDA-MB-453s using 11962-1-AP (same clone as 11962-1-PBS)

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11962-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using MCPH/BRIT1 Polyclonal antibody (11962-1-AP)

    WB analysis of L02 using 11962-1-AP (same clone as 11962-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11962-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDA-MB-453s cells using MCPH/BRIT1 Polyclonal antibody (11962-1-AP)

    WB analysis of MDA-MB-453s using 11962-1-AP (same clone as 11962-1-PBS)

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11962-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDA-MB-453s cells using MCPH/BRIT1 Polyclonal antibody (11962-1-AP)

    WB analysis of MDA-MB-453s using 11962-1-AP (same clone as 11962-1-PBS)

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11962-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDA-MB-453s cells using MCPH/BRIT1 Polyclonal antibody (11962-1-AP)

    WB analysis of MDA-MB-453s using 11962-1-AP (same clone as 11962-1-PBS)

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11962-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse ovary tissue using MCPH/BRIT1 Polyclonal antibody (11962-1-AP)

    WB analysis of mouse ovary using 11962-1-AP (same clone as 11962-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11962-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MCPH/BRIT1 Polyclonal antibody (11962-1-AP)

    IHC staining of human prostate cancer using 11962-1-AP (same clone as 11962-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11962-1-AP (BRIT1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11962-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MCPH/BRIT1 Polyclonal antibody (11962-1-AP)

    IHC staining of human kidney using 11962-1-AP (same clone as 11962-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 11962-1-AP (BRIT1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11962-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11962-1-PBS targets MCPH/BRIT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCPH/BRIT1 fusion protein Ag2567 相同性解析による交差性が予測される生物種
Full Name microcephalin 1
Calculated molecular weight 93 kDa
Observed molecular weight 66 kDa, 100 kDa
GenBank accession numberBC030702
Gene symbol MCPH1
Gene ID (NCBI) 79648
RRIDAB_2142989
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Primary microcdphaly refers to the clinical finding of a head circumference less than 3 standard deviations(SD) below the age- and sex-related mean, present at birth. MCPH1, the fisrt gene identified as causative for primary microcephaly, encodes a multifunctional protein that notably is linked to DNA damage checkpoint, DNA repair by homologous recombination and DNA transcription. MCPH1 is associated with premature chromosome condensation in particular. There are three mainly isoforms encoded by MCPH1 gene, full-length MCPH1 (MCPH1-FL) (~100kd), lacking the C-terminal BRCTisoform 2(~70kd) and lacking middle region isoform 3(~50kd).(PMID: 22952573 )