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Species Reactivity
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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated MDC1 Recombinant antibody

MDC1 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240469D5

Cat no : CL488-83496

Synonyms

NFBD1, NFBD 1, MDC 1, 240469D5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated MDC1 Recombinant ant (CL488-83496)

    IF Staining of HeLa using CL488-83496

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 MDC1 antibody (CL488-83496, Clone: 240469D5 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated MDC1 Recombinant ant (CL488-83496)

    FC experiment of HeLa using CL488-83496

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated MDC1 Recombinant antibody (CL488-83496, Clone:240469D5)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83496 is MDC1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MDC1 fusion protein Ag20135 相同性解析による交差性が予測される生物種
Full Name mediator of DNA damage checkpoint 1
Calculated molecular weight 2089 aa, 227 kDa
Observed molecular weight260-320 kDa
GenBank accession numberBC152556
Gene symbol MDC1
Gene ID (NCBI) 9656
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MDC1 antibody CL488-83496Download protocol
FC protocol for CL Plus 488 MDC1 antibody CL488-83496Download protocol
Standard Protocols
Click here to view our Standard Protocols