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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MDH1 Recombinant antibody, PBS Only

MDH1 Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241887A1

Cat no : 84576-4-PBS

Synonyms

MDHA, KAR, EC:1.1.1.96, EC:1.1.1.37, Aromatic alpha-keto acid reductase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MDH1 Recombinant antibody (84576-4-RR)

    WB analysis using 84576-4-RR (same clone as 84576-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84576-4-RR (MDH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84576-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MDH1 Recombinant antibody (84576-4-RR)

    IHC staining of mouse heart using 84576-4-RR (same clone as 84576-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84576-4-RR (MDH1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84576-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MDH1 Recombinant antibody (84576-4-RR)

    IHC staining of mouse heart using 84576-4-RR (same clone as 84576-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84576-4-RR (MDH1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84576-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84576-4-RR

    Affinity and Kinetic Characterization of 84576-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84576-4-RR against Human MDH1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84576-4-PBS targets MDH1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MDH1 fusion protein Ag8744 相同性解析による交差性が予測される生物種
Full Name malate dehydrogenase 1, NAD (soluble)
Calculated molecular weight 334 aa, 36 kDa
Observed molecular weight 35-37 kDa
GenBank accession numberBC001484
Gene symbol MDH1
Gene ID (NCBI) 4190
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme.(PMID:10693743)