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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MDH1 Recombinant antibody

MDH1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241887A1

Cat no : 84576-4-RR

Synonyms

MDHA, KAR, EC:1.1.1.96, EC:1.1.1.37, Aromatic alpha-keto acid reductase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MDH1 Recombinant antibody (84576-4-RR)

    WB analysis using 84576-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84576-4-RR (MDH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HL-60 cells, mouse liver tissue, rat liver tissue, mouse kidney tissue, human kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84576-4-RR is MDH1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MDH1 fusion protein Ag8744 相同性解析による交差性が予測される生物種
Full Name malate dehydrogenase 1, NAD (soluble)
Calculated molecular weight 334 aa, 36 kDa
Observed molecular weight 35-37 kDa
GenBank accession numberBC001484
Gene symbol MDH1
Gene ID (NCBI) 4190
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme.(PMID:10693743)

Protocols

Product Specific Protocols
WB protocol for MDH1 antibody 84576-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols