Validation Data Gallery
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Tested Applications
| Positive WB detected in | Raji cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL750-83141-2 targets MDM1 in WB applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | MDM1 fusion protein Ag10541 相同性解析による交差性が予測される生物種 |
| Full Name | Mdm1 nuclear protein homolog (mouse) |
| Calculated molecular weight | 81 kDa |
| Observed molecular weight | 102 kDa |
| GenBank accession number | BC028355 |
| Gene Symbol | MDM1 |
| Gene ID (NCBI) | 56890 |
| RRID | AB_3673772 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8TC05 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mdm1 was upregulated during ciliogenesis in multiciliated tracheal epithelial cells and localized to centrioles and cilia when transfected into NIH/3T3 cells. Mdm1 is a microtubule-binding protein that inhibits centrosome duplication when overexpressed. Proteomic analysis revealed that Mdm1 was also detected in the mouse photoreceptor sensory cilium complex and the outer segment (OS) of photoreceptor cells in the retina, suggesting that Mdm1 might be involved in the regulation of the functions and structure of the centrosome, as well as cilia. (PMID: 36171205)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 MDM1 antibody CL750-83141-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |