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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ME1 Monoclonal antibody

ME1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2G9G9

Cat no : 68117-1-Ig

Synonyms

NADP-ME, NADP-dependent malic enzyme, Malic enzyme 1, EC:1.1.1.40, 2G9G9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ME1 Monoclonal antibody (68117-1-Ig)

    WB analysis using 68117-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68117-1-Ig (ME1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using ME1 Monoclonal antibody (68117-1-Ig)

    WB analysis using 68117-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68117-1-Ig (ME1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ME1 Monoclonal antibody (68117-1-Ig)

    IF Staining of HepG2 using 68117-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ME1 antibody (68117-1-Ig, Clone: 2G9G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Flow cytometry (FC) experiment of HeLa cells using ME1 Monoclonal antibody (68117-1-Ig)

    FC experiment of HeLa using 68117-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug ME1 Monoclonal antibody (68117-1-Ig, Clone:2G9G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA549 cells, pig brain tissue, HCT 116 cells, LNCaP cells, HeLa cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68117-1-Ig targets ME1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ME1 fusion protein Ag9916 相同性解析による交差性が予測される生物種
Full Name malic enzyme 1, NADP(+)-dependent, cytosolic
Calculated molecular weight 572 aa, 64 kDa
Observed molecular weight55-64 kDa
GenBank accession numberBC025246
Gene symbol ME1
Gene ID (NCBI) 4199
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ME1(NADP-dependent malic enzyme) belongs to the malic enzymes family. This malic enzyme catalyzes the reversible oxidative decarboxylation of malate and is a link between the glycolytic pathway and the citric acid cycle. The reaction is L-malate plus NADP(+) to form pyruvate, CO(2), and NADPH.

Protocols

Product Specific Protocols
WB protocol for ME1 antibody 68117-1-IgDownload protocol
IF protocol for ME1 antibody 68117-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols