ME1 Monoclonal antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 68117-1-Ig (ME1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68117-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 68117-1-Ig (ME1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68117-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ME1 antibody (68117-1-Ig, Clone: 2G9G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68117-1-PBS in a different storage buffer formulation.

1x10^6 HeLa cells were intracellularly stained with 0.8 ug ME1 Monoclonal antibody (68117-1-Ig, Clone:2G9G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68117-1-PBS in a different storage buffer formulation.