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  • KD/KO Validated

MED15 Polyclonal antibody

MED15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 11566-1-AP

Synonyms

Mediator of RNA polymerase II transcription subunit 15, CTG7A, CTG repeat protein 7a, CAG7A, ARC105


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Tested Applications

Positive WB detected inK-562 cells, SMMC-7721 cells, HuH-7 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11566-1-AP targets MED15 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MED15 fusion protein Ag2143 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 15
Calculated molecular weight 788 aa, 87 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC017110
Gene symbol MED15
Gene ID (NCBI) 51586
RRIDAB_2141975
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MED15, also named as TIG1, CAG7A, CTG7A, PCQAP, TIG-1, TNRC7 and ARC105, is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. It contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.MED15 is recruited to promoters by direct interaction with gene-specific regulatory proteins. It is about 105kd in SDS-page(doi.org/10.3109/10409238.2013.840259).

Protocols

Product Specific Protocols
WB protocol for MED15 antibody 11566-1-APDownload protocol
IHC protocol for MED15 antibody 11566-1-APDownload protocol
IF protocol for MED15 antibody 11566-1-APDownload protocol
IP protocol for MED15 antibody 11566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Cell

Tyrosine-1 of RNA Polymerase II CTD Controls Global Termination of Gene Transcription in Mammals.

Authors - Nilay Shah
mouseWB

Cell Rep

Mediator MED23 Links Pigmentation and DNA Repair through the Transcription Factor MITF.

Authors - Min Xia
humanWB,IHC,IF

Int J Cancer

MED15, encoding a subunit of the mediator complex, is overexpressed at high frequency in castration-resistant prostate cancer.

Authors - Zaki Shaikhibrahim
  • KD Validated
human, mouseChIP

PLoS Genet

Mediator subunit MDT-15/MED15 and Nuclear Receptor HIZR-1/HNF4 cooperate to regulate toxic metal stress responses in Caenorhabditis elegans.

Authors - Naomi Shomer
humanWB

J Biol Chem

Mediator complex recruits epigenetic regulators via its two cyclin-dependent kinase subunits to repress transcription of immune-response genes.

Authors - Tsutsui Taiki T
humanWB, IHC, IF

Am J Pathol

Clinical and Molecular Implications of MED15 in Head and Neck Squamous Cell Carcinoma.

Authors - Zaki Shaikhibrahim

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

SITING (Verified Customer) (04-02-2021)

between 75-100

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: EMBRYO HEART
MED15 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:11566-1-AP)