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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite® Plus 488-conjugated MED15 Polyclonal ant (CL488-11566)

    IF Staining of SH-SY5Y using CL488-11566

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 MED15 antibody (CL488-11566) at dilution of 1:100.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MED15 Polyclonal antibody

MED15 Polyclonal Antibody for IF/ICC
Cat No. CL488-11566

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

ARC105, CAG7A, CTG repeat protein 7a, CTG7A, DKFZp686A2214, DKFZp762B1216, FLJ42282, FLJ42935, MED15, mediator complex subunit 15, PCQAP, TIG 1, TIG1, TNRC7, TPA inducible gene 1 protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11566 targets MED15 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MED15 fusion protein Ag2143 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 15
Calculated molecular weight 788 aa, 87 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC017110
Gene Symbol MED15
Gene ID (NCBI) 51586
RRIDAB_3083905
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RN5
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MED15, also named as TIG1, CAG7A, CTG7A, PCQAP, TIG-1, TNRC7 and ARC105, is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. It contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.MED15 is recruited to promoters by direct interaction with gene-specific regulatory proteins. It is about 105kd in SDS-page(doi.org/10.3109/10409238.2013.840259).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MED15 antibody CL488-11566Download protocol
Standard Protocols
Click here to view our Standard Protocols