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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using MED15 Polyclonal antibody (11566-1-AP)

    WB analysis of K-562 using 11566-1-AP (same clone as 11566-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SMMC-7721 cells using MED15 Polyclonal antibody (11566-1-AP)

    WB analysis of SMMC-7721 using 11566-1-AP (same clone as 11566-1-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HuH-7 cells using MED15 Polyclonal antibody (11566-1-AP)

    WB analysis of HuH-7 using 11566-1-AP (same clone as 11566-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using MED15 Polyclonal antibody (11566-1-AP)

    IP experiment of K-562 using 11566-1-AP (same clone as 11566-1-PBS)

    IP result of anti-MED15 (IP:11566-1-AP, 3ug; Detection:11566-1-AP 1:500) with K-562 cells lysate 3440ug. This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MED15 Polyclonal antibody (11566-1-AP)

    IHC staining of human prostate cancer using 11566-1-AP (same clone as 11566-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11566-1-AP (MED15 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using MED15 Polyclonal antibody (11566-1-AP)

    IF Staining of SH-SY5Y using 11566-1-AP (same clone as 11566-1-PBS)

    Immunofluorescent analysis of SH-SY5Y cells using 11566-1-AP (MED15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

MED15 Polyclonal antibody, PBS Only

MED15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11566-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11566-1-PBS targets MED15 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MED15 fusion protein Ag2143 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 15
Calculated molecular weight 788 aa, 87 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC017110
Gene Symbol MED15
Gene ID (NCBI) 51586
RRIDAB_2141975
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RN5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MED15, also named as TIG1, CAG7A, CTG7A, PCQAP, TIG-1, TNRC7 and ARC105, is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. It contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.MED15 is recruited to promoters by direct interaction with gene-specific regulatory proteins. It is about 105kd in SDS-page(doi.org/10.3109/10409238.2013.840259).