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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MED21 Recombinant antibody (83510-1-RR)

    WB analysis of HepG2 using 83510-1-RR (same clone as 83510-1-PBS)

    WB result of MED21 antibody (83510-1-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-MED21 transfected HepG2 cells. This data was developed using the same antibody clone with 83510-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MED21 Recombinant antibody (83510-1-RR)

    WB analysis using 83510-1-RR (same clone as 83510-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83510-1-RR (MED21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83510-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MED21 Recombinant antibody (83510-1-RR)

    FC experiment of HeLa using 83510-1-RR (same clone as 83510-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MED21 Recombinant antibody (83510-1-RR, Clone:240419E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83510-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-87 MG cells using MED21 Recombinant antibody (83510-1-RR)

    FC experiment of U-87 MG using 83510-1-RR (same clone as 83510-1-PBS)

    1x10^6 U-87 MG cells were intracellularly stained with 0.25 ug MED21 Recombinant antibody (83510-1-RR, Clone:240419E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83510-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00506-1

    Cytometric bead array standard curve of MP00506-1

    Cytometric bead array standard curve of MP00506-1, MED21 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83510-1-PBS. Detection antibody: 83510-2-PBS. Standard: Ag8568. Range: 1.25-40 ng/mL.

  • Affinity and Kinetic Characterization of 83510-1-RR

    Affinity and Kinetic Characterization of 83510-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83510-1-RR against Human MED21 were performed. The affinity constant is 2.06 nM.

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  • KD/KO Validated

MED21 Recombinant antibody, PBS Only (Capture)

MED21 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83510-1-PBS
Clone No.240419E2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

SURB7, SRB7, hSrb7, 240419E2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83510-1-PBS targets MED21 as part of a matched antibody pair:

MP00506-1: 83510-1-PBS capture and 83510-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MED21 fusion protein Ag8568 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 21
Calculated molecular weight 144 aa, 16 kDa
Observed molecular weight13-16 kDa
GenBank accession numberBC008380
Gene Symbol MED21
Gene ID (NCBI) 9412
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13503
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MED21, also known as SRB7 and SURB7,belongs to the Mediator complex subunit 21 family. MED21 is a small essential subunit of the middle domain of the Mediator, which is conserved in all eukaryotes. MED21 is thought to play an important role in transcriptional activation and repression. MED21 regulates the transcription of nearly all RNA polymerase II-dependent genes (PMID: 16758199, PMID: 30559736).