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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MED21 Recombinant antibody (83510-1-RR)

    WB analysis of HepG2 using 83510-1-RR

    WB result of MED21 antibody (83510-1-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-MED21 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using MED21 Recombinant antibody (83510-1-RR)

    WB analysis using 83510-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83510-1-RR (MED21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using MED21 Recombinant antibody (83510-1-RR)

    FC experiment of HeLa using 83510-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MED21 Recombinant antibody (83510-1-RR, Clone:240419E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of U-87 MG cells using MED21 Recombinant antibody (83510-1-RR)

    FC experiment of U-87 MG using 83510-1-RR

    1x10^6 U-87 MG cells were intracellularly stained with 0.25 ug MED21 Recombinant antibody (83510-1-RR, Clone:240419E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83510-1-RR

    Affinity and Kinetic Characterization of 83510-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83510-1-RR against Human MED21 were performed. The affinity constant is 2.06 nM.

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  • Featured Product
  • KD/KO Validated

MED21 Recombinant antibody

MED21 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83510-1-RR
Clone No.240419E2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

SURB7, SRB7, hSrb7, 240419E2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, L02 cells, HEK-293T cells
Positive FC (Intra) detected inHeLa cells, U-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83510-1-RR targets MED21 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MED21 fusion protein Ag8568 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 21
Calculated molecular weight 144 aa, 16 kDa
Observed molecular weight13-16 kDa
GenBank accession numberBC008380
Gene Symbol MED21
Gene ID (NCBI) 9412
RRIDAB_3671135
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13503
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MED21, also known as SRB7 and SURB7,belongs to the Mediator complex subunit 21 family. MED21 is a small essential subunit of the middle domain of the Mediator, which is conserved in all eukaryotes. MED21 is thought to play an important role in transcriptional activation and repression. MED21 regulates the transcription of nearly all RNA polymerase II-dependent genes (PMID: 16758199, PMID: 30559736).

Protocols

Product Specific Protocols
WB protocol for MED21 antibody 83510-1-RRDownload protocol
FC protocol for MED21 antibody 83510-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols