MED23 Monoclonal antibody

MED23 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H4G4

Cat no : 68307-1-Ig

Synonyms

ARC130, CRSP complex subunit 3, CRSP130, CRSP133, CRSP3, DKFZp434H0117, DRIP130, hSur 2, KIAA1216, MED23, mediator complex subunit 23, Protein sur 2 homolog, SUR2


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Tested Applications

Positive WB detected inA549 cells, 4T1 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68307-1-Ig targets MED23 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MED23 fusion protein Ag28483 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 23
Calculated molecular weight 156 kDa
Observed molecular weight150 kDa
GenBank accession numberBC060759
Gene symbol MED23
Gene ID (NCBI) 9439
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MED23 is a component of the Mediator complex, it is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.

Protocols

Product Specific Protocols
WB protocol for MED23 antibody 68307-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols