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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MED23 Monoclonal antibody (68307-1-Ig)

    WB analysis using 68307-1-Ig (same clone as 68307-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68307-1-Ig (MED23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68307-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using MED23 Monoclonal antibody (68307-1-Ig)

    WB analysis of 4T1 using 68307-1-Ig (same clone as 68307-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 68307-1-Ig (MED23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68307-1-PBS in a different storage buffer formulation.

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MED23 Monoclonal antibody, PBS Only

MED23 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68307-1-PBS
Clone No.1H4G4

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Synonyms

ARC130, CRSP complex subunit 3, CRSP130, CRSP133, CRSP3, DKFZp434H0117, DRIP130, hSur 2, KIAA1216, MED23, mediator complex subunit 23, Protein sur 2 homolog, SUR2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68307-1-PBS targets MED23 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MED23 fusion protein Ag28483 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 23
Calculated molecular weight 156 kDa
Observed molecular weight150 kDa
GenBank accession numberBC060759
Gene Symbol MED23
Gene ID (NCBI) 9439
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9ULK4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MED23 is a component of the Mediator complex, it is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.