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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MED30 Monoclonal antibody

MED30 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D7C4

Cat no : CL488-67038

Synonyms

Thyroid hormone receptor-associated protein complex 25 kDa component, Thyroid hormone receptor-associated protein 6, THRAP6, Mediator of RNA polymerase II transcription subunit 30, Mediator complex subunit 30

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated MED30 Monoclonal ant (CL488-67038)

    FC experiment of HeLa using CL488-67038

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MED30 (CL488-67038, Clone:1D7C4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67038 targets MED30 in FC (Intra) applications and shows reactivity with Human, rat, pig samples.

Tested Reactivity Human, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MED30 fusion protein Ag10235 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 30
Calculated molecular weight 178 aa, 20 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC008226
Gene symbol MED30
Gene ID (NCBI) 90390
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MED30, also named as THRAP6 and TRAP25, is a component of the Mediator complex. MED30 has two isoforms with MW 16-20 kDa ad 20-25 kDa.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 MED30 antibody CL488-67038Download protocol
Standard Protocols
Click here to view our Standard Protocols