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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated MED4 Monoclonal anti (CL488-67839)

    FC experiment of HepG2 using CL488-67839

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MED4 (CL488-67839, Clone:2E6F4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite® Plus 488-conjugated MED4 Monoclonal antibody

MED4 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67839
Clone No.2E6F4

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, rat

Applications

FC (Intra)

Synonyms

MED4, Mediator of RNA polymerase II transcription subunit 4, TRAP36, VDRIP, Vitamin D3 receptor-interacting protein complex 36 kDa component, 2E6F4, Activator-recruited cofactor 36 kDa component, ARC36, DRIP36, HSPC126

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67839 targets MED4 in FC (Intra) applications and shows reactivity with Human, Mouse, rat samples.

Tested Reactivity Human, Mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MED4 fusion protein Ag8562 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 4
Calculated molecular weight 270 aa, 30 kDa
Observed molecular weight~36 kDa
GenBank accession numberBC005189
Gene Symbol MED4
Gene ID (NCBI) 29079
RRIDAB_2934573
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NPJ6
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MED4 is a component of the Meditor complex, which is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Meditor functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. It also have an alternative name called "Activator-recruited cofactor 36 kDa component (ARC36) ".

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 MED4 antibody CL488-67839Download protocol
Standard Protocols
Click here to view our Standard Protocols