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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MED6 Recombinant antibody (84184-5-RR)

    IF Staining of A431 using 84184-5-RR (same clone as 84184-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MED6 antibody (84184-5-RR, Clone: 241382B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84184-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84184-5-RR

    Affinity and Kinetic Characterization of 84184-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84184-5-RR against Human MED6 were performed. The affinity constant is below 1 pM.

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MED6 Recombinant antibody, PBS Only

MED6 Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA
Cat No. 84184-5-PBS
Clone No.241382B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Synonyms

NY REN 28, Mediator of RNA polymerase II transcription subunit 6, hMed6, ARC33, Activator-recruited cofactor 33 kDa component

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84184-5-PBS targets MED6 in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MED6 fusion protein Ag7541 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 6
Calculated molecular weight 28 kDa
GenBank accession numberBC004106
Gene Symbol MED6
Gene ID (NCBI) 10001
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO75586
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.