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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MED6 Recombinant antibody

MED6 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241382B2

Cat no : 84184-5-RR

Synonyms

NY REN 28, Mediator of RNA polymerase II transcription subunit 6, hMed6, ARC33, Activator-recruited cofactor 33 kDa component

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MED6 Recombinant antibody (84184-5-RR)

    IF Staining of A431 using 84184-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MED6 antibody (84184-5-RR, Clone: 241382B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84184-5-RR

    Affinity and Kinetic Characterization of 84184-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84184-5-RR against Human MED6 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84184-5-RR is MED6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MED6 fusion protein Ag7541 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 6
Calculated molecular weight 28 kDa
GenBank accession numberBC004106
Gene symbol MED6
Gene ID (NCBI) 10001
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Protocols

Product Specific Protocols
IF protocol for MED6 antibody 84184-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols