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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MEK1/2 Polyclonal antibody (11049-1-AP)

    WB analysis using 11049-1-AP (same clone as 11049-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MEK1/2 Polyclonal antibody (11049-1-AP)

    WB analysis using 11049-1-AP (same clone as 11049-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using MEK1/2 Polyclonal antibody (11049-1-AP)

    WB analysis of human brain using 11049-1-AP (same clone as 11049-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11049-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HL-60 cells using MEK1/2 Polyclonal antibody (11049-1-AP)

    IP experiment of HL-60 using 11049-1-AP (same clone as 11049-1-PBS)

    IP result of anti-MEK1/2 (IP:11049-1-AP, 4ug; Detection:11049-1-AP 1:1000) with HL-60 cells lysate 1280ug. This data was developed using the same antibody clone with 11049-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MEK1/2 Polyclonal antibody (11049-1-AP)

    IHC staining of mouse brain using 11049-1-AP (same clone as 11049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11049-1-AP (MEK1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11049-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MEK1/2 Polyclonal antibody (11049-1-AP)

    IHC staining of mouse brain using 11049-1-AP (same clone as 11049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11049-1-AP (MEK1/2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11049-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using MEK1/2 Polyclonal antibody (11049-1-AP)

    IF Staining of Jurkat using 11049-1-AP (same clone as 11049-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MEK1/2 antibody (11049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11049-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using MEK1/2 Polyclonal antibody (11049-1-AP)

    IF Staining of SH-SY5Y using 11049-1-AP (same clone as 11049-1-PBS)

    Immunofluorescent analysis of SH-SY5Y cells using 11049-1-AP (MEK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11049-1-PBS in a different storage buffer formulation.

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MEK1/2 Polyclonal antibody, PBS Only

MEK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11049-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11049-1-PBS targets MEK1/2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEK1/2 fusion protein Ag1481 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 2
Calculated molecular weight 44 kDa
Observed molecular weight 44-47 kDa
GenBank accession numberBC018645
Gene Symbol MEK2
Gene ID (NCBI) 5605
RRIDAB_2140649
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36507
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 11049-1-AP can recognize both the MEK2 and MEK1.