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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MEK5 Polyclonal antibody, PBS Only

MEK5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15758-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293T cells using MEK5 Polyclonal antibody (15758-1-AP)

    WB analysis of HEK-293T using 15758-1-AP (same clone as 15758-1-PBS)

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 15758-1-AP (MEK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15758-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using MEK5 Polyclonal antibody (15758-1-AP)

    WB analysis of A431 using 15758-1-AP (same clone as 15758-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 15758-1-AP (MEK5 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15758-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MEK5 Polyclonal antibody (15758-1-AP)

    WB analysis of HeLa using 15758-1-AP (same clone as 15758-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15758-1-AP (MEK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15758-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MEK5 Polyclonal antibody (15758-1-AP)

    IHC staining of human heart using 15758-1-AP (same clone as 15758-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15758-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MEK5 Polyclonal antibody (15758-1-AP)

    IHC staining of human heart using 15758-1-AP (same clone as 15758-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15758-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using MEK5 Polyclonal antibody (15758-1-AP)

    IHC staining of human testis using 15758-1-AP (same clone as 15758-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15758-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using MEK5 Polyclonal antibody (15758-1-AP)

    IHC staining of human testis using 15758-1-AP (same clone as 15758-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15758-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using MEK5 Polyclonal antibody (15758-1-AP)

    IHC staining of human testis using 15758-1-AP (same clone as 15758-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 15758-1-AP (MEK5 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15758-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using MEK5 Polyclonal antibody (15758-1-AP)

    IHC staining of human testis using 15758-1-AP (same clone as 15758-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 15758-1-AP (MEK5 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15758-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MEK5 Polyclonal antibody (15758-1-AP)

    IF Staining of HeLa using 15758-1-AP (same clone as 15758-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEK5 antibody (15758-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15758-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15758-1-PBS targets MEK5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEK5 fusion protein Ag8351 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 5
Calculated molecular weight 448 aa, 50 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC008838
Gene symbol MEK5
Gene ID (NCBI) 5607
RRIDAB_2141275
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.