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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MEN1 Polyclonal antibody

MEN1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15159-1-AP

Synonyms

SCG2, Menin 1, Menin, MEAI

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MEN1 Polyclonal antibody (15159-1-AP)

    WB analysis of HepG2 using 15159-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15159-1-AP (MEN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MEN1 Polyclonal antibody (15159-1-AP)

    WB analysis of Jurkat using 15159-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15159-1-AP (MEN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MEN1 Polyclonal antibody (15159-1-AP)

    WB analysis of HeLa using 15159-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15159-1-AP (MEN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using MEN1 Polyclonal antibody (15159-1-AP)

    IP experiment of HEK-293 using 15159-1-AP

    IP result of anti-MEN1 (IP:15159-1-AP, 4ug; Detection:15159-1-AP 1:500) with HEK-293 cells lysate 1320 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MEN1 Polyclonal antibody (15159-1-AP)

    IF Staining of HeLa using 15159-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15159-1-AP (MEN1 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15159-1-AP targets MEN1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEN1 fusion protein Ag7253 相同性解析による交差性が予測される生物種
Full Name multiple endocrine neoplasia I
Calculated molecular weight 68 kDa
Observed molecular weight 63-70 kDa
GenBank accession numberBC002544
Gene symbol MEN1
Gene ID (NCBI) 4221
RRIDAB_2250607
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEN1, also named as Menin or MEAI, is a tumor suppressor protein encoded by the MEN1 gene. Its inactivation has been implicated in tumors of many endocrine tissues. Germline inactivating mutations of MEN1 gene are the main cause of multiple endocrine neoplasia type 1 (MEN1), a syndrome characterized by tumors in the pituitary gland, the parathyroid glands, and the enteropancreatic neuroendocrine tissues. Recent study shows astroglia menin deficiency leads to depressive-like behaviors in mice

Protocols

Product Specific Protocols
WB protocol for MEN1 antibody 15159-1-APDownload protocol
IF protocol for MEN1 antibody 15159-1-APDownload protocol
IP protocol for MEN1 antibody 15159-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

DOT1L/H3K79me2 represses HIV-1 reactivation via recruiting DCAF1

Authors - Fenfei Liang
View Article