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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

METTL1 Monoclonal antibody

METTL1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C3G7

Cat no : 68501-1-Ig

Synonyms

C12orf1, FLJ95748, methyltransferase like 1, METTL1, TRM8, tRNA(m7G46) methyltransferase, YDL201w

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL1 Monoclonal antibody (68501-1-Ig)

    WB analysis using 68501-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68501-1-Ig (METTL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using METTL1 Monoclonal antibody (68501-1-Ig)

    WB analysis using 68501-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68501-1-Ig (METTL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inLNCaP cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68501-1-Ig targets METTL1 in WB, ELISA applications and shows reactivity with Human, mouse, pig samples.

Tested Reactivity Human, mouse, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen METTL1 fusion protein Ag7055 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 1
Calculated molecular weight 31 kDa
Observed molecular weight31 kDa
GenBank accession numberBC000550
Gene symbol METTL1
Gene ID (NCBI) 4234
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.

Protocols

Product Specific Protocols
WB protocol for METTL1 antibody 68501-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols