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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

METTL6 Monoclonal antibody

METTL6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C7D4

Cat no : 68400-1-Ig

Synonyms

methyltransferase like 6, METTL6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL6 Monoclonal antibody (68400-1-Ig)

    WB analysis using 68400-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68400-1-Ig (METTL6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using METTL6 Monoclonal antibody (68400-1-Ig)

    IHC staining of human breast cancer using 68400-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using METTL6 Monoclonal antibody (68400-1-Ig)

    IHC staining of human lung cancer using 68400-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using METTL6 Monoclonal antibody (68400-1-Ig)

    IHC staining of human lung cancer using 68400-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using METTL6 Monoclonal antibody (68400-1-Ig)

    IHC staining of human breast cancer using 68400-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68400-1-Ig targets METTL6 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen METTL6 fusion protein Ag9714 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 6
Calculated molecular weight 255 aa, 30 kDa
Observed molecular weight 30-32 kDa
GenBank accession numberBC022400
Gene symbol METTL6
Gene ID (NCBI) 131965
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent nontumor tissues. CRISPR/Cas9mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).

Protocols

Product Specific Protocols
WB protocol for METTL6 antibody 68400-1-IgDownload protocol
IHC protocol for METTL6 antibody 68400-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols