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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL6 Monoclonal antibody (68400-1-Ig)

    WB analysis using 68400-1-Ig (same clone as 68400-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68400-1-Ig (METTL6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using METTL6 Monoclonal antibody (68400-1-Ig)

    IHC staining of human breast cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using METTL6 Monoclonal antibody (68400-1-Ig)

    IHC staining of human lung cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using METTL6 Monoclonal antibody (68400-1-Ig)

    IHC staining of human lung cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using METTL6 Monoclonal antibody (68400-1-Ig)

    IHC staining of human breast cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

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METTL6 Monoclonal antibody, PBS Only

METTL6 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68400-1-PBS
Clone No.1C7D4

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

methyltransferase like 6, METTL6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

68400-1-Ig

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68400-1-PBS targets METTL6 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen METTL6 fusion protein Ag9714 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 6
Calculated molecular weight 255 aa, 30 kDa
Observed molecular weight 30-32 kDa
GenBank accession numberBC022400
Gene Symbol METTL6
Gene ID (NCBI) 131965
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TCB7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent nontumor tissues. CRISPR/Cas9mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).